The continuing threats of military conflicts and terrorism may involve the misuse of chemical weapons. Despite the effort of the Organisation for the Prohibition of Chemical Weapons (OPCW), chemical weapons are regularly used, e.g. in the Syrian Arab Republic [1]. If there is an investigation of alleged use of these toxic chemicals, environmental and biomedical samples are taken. Subsequently, forensic investigations are performed to identify the chemical and to apply impurity profiling for intelligence purposes.

One of the remaining challenges in chemical impurity profiling concerns the stability of the actual sample during sampling, transportation, storage and analysis [2]. Especially in case of reactive chemicals such as chemical warfare agents (CWAs) it can be expected that a specific profile is highly prone towards change over time, in particular when some of the components remain in their reactive state. A possible way of ‘freezing’ the signature might be through drying plasma or whole blood on filter paper [3,4]. For instance, a sulfur mustard adduct in human serum albumin is a very stable biomarker in plasma samples even weeks after exposure [5]. This fixation will transform at least the reactive components in a more stable form and will also stop possible degradation of other components within the chemical profile.

"One of the remaining challenges in chemical impurity profiling concerns the stability of the sample."

The aim of this literature thesis is to explore whether dried blood spots can be used to maintain a stable attribution signature of chemical warfare agents. Subsequently, various sample preparation strategies can be investigated which are required for the analytical chemical analysis.

Apply for a literature thesis on this subject. You can find more information at: open_in_new clhc.nl

References

[1] United Nations Mission to Investigate Allegations of the Use of Chemical Weapons in the Syrian Arab Republic, Final report, 2013.

[2] E.I. Hamelin, T.A. Blake, J.W. Perez, B.S. Crow, R.L. Shaner, R.M. Coleman, R.C. Johnson, Bridging the gap between sample collection and laboratory analysis: using dried blood spots to identify human exposure to chemical agents, Smart Biomed. Physiol. Sens. Technol. XIII. 9863 (2016) 98630P. https://doi.org/10.1117/12.2223796.

[3] J.W. Perez, B.G. Pantazides, C.M. Watson, J.D. Thomas, T.A. Blake, R.C. Johnson, Enhanced stability of blood matrices using a dried sample spot assay to measure human butyrylcholinesterase activity and nerve agent adducts, Anal. Chem. 87 (2015) 5723–5729. https://doi.org/10.1021/acs.analchem.5b00893.

[4] R.L. Shaner, R.M. Coleman, N. Schulze, K. Platanitis, A.A. Brown, C. Seymour, P. Kaplan, J. Perez, E.I. Hamelin, R.C. Johnson, Investigation of dried blood sampling with liquid chromatography tandem mass spectrometry to confirm human exposure to nerve agents, Anal. Chim. Acta. 1033 (2018) 100–107. https://doi.org/10.1016/j.aca.2018.06.049.

[5] AH. John, S. Willoh, P. Hörmann, M. Siegert, A. Vondran, H. Thiermann, Procedures for Analysis of Dried Plasma Using Microsampling Devices to Detect Sulfur Mustard-Albumin Adducts for Verification of Poisoning, Anal. Chem. 88 (2016) 8787–8794. https://doi.org/10.1021/acs.analchem.6b02199.

[6] E. Underwood, A. Cho, News this Week, Science (80-. ). 342 (2013) 540.